Composite
λcI-x

Part:BBa_K2717023

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


λcI-xiao(λ repressor-lac operator-GFP)

In this composited part, we link GFP with upstream CI to control the whole coding. We design this part to test the efficiency of growth activation of CI protein. And we first use the sequence in part with the promoter from BBa_K1139150.

function:We design this part to test the efficiency of growth activation of CI protein. The improvement of green fluorescence can be detected under the induction of IPTG. Comparing with control, CI protein has the ability to improve the expression of downstream protein by 150%.

T--BNU-China--_R18.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 766
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 742
    Illegal BsaI.rc site found at 1540


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Parameters
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